The four groups of mice differed greatly in their activity levels before treatment but showed identical activity levels at the end of the monitoring period.

Saline-treated females showed substantially more activity at certain points in the monitoring period than saline-treated males did.

CNO-treated females showed more activity relative to saline-treated females than CNO-treated males showed relative to saline-treated males.

CNO-treated females showed a substantial increase and then decline in activity over the monitoring period, whereas CNO-treated males showed a substantial decline in activity followed by a steep increase.

English

PASSAGE: To investigate the influence of certain estrogen-responsive neurons on energy expenditure, biologist Stephanie Correa et al. treated female and male mice with either saline solution or clozapine-N4-oxide (CNO), which activates the neurons. Monitoring the activity levels of the mice by measuring how frequently the animals broke infrared beams crossing their enclosures, Correa et al. found that the mice in their study showed sex-specific differences in response to neuron activation: ____

 QUESTION: Which choice most effectively uses data from the graph to complete the assertion?

